Journal: Biochemistry and Biophysics Reports

Article Title: Investigating the ABTS-based antioxidant potential and antiproliferative activity of pulp, skin and seeds extracts from Nephelium lappaceum , Manilkara zapota , and Meliccocus oliviformis on human prostate and colon cancer cells

doi: 10.1016/j.bbrep.2025.102257

Figure Lengend Snippet: Effects of the different fruit extracts on the proliferation of the colon cancer cell line HCT116 and the prostate cancer cell line DU145 . DU145 (A) or HCT116 (B) were seeded in a 12-well plate and allowed to grow for 12 h. Then, 500 μg/mLof PBS-resuspended extract derived from the pulp, seeds, and skin of Meliccocus oliviformis , Nephelium lappaceum , and Manilkara zapota were added and cell proliferation was monitored by analyzing the surface area occupied by the cells in each well (% confluence) from photos taken at regular intervals (every 3 h) by the Incucyte live cell imaging system over a period of three days. Data shown are the average ± SD of three independent experiments.

Article Snippet: The HCT116 (colic), and DU145 (prostatic) human cancer cell lines were purchased from ATCC (American Type Culture Collection).

Techniques: Derivative Assay, Live Cell Imaging

Journal: Biochemistry and Biophysics Reports

Article Title: Investigating the ABTS-based antioxidant potential and antiproliferative activity of pulp, skin and seeds extracts from Nephelium lappaceum , Manilkara zapota , and Meliccocus oliviformis on human prostate and colon cancer cells

doi: 10.1016/j.bbrep.2025.102257

Figure Lengend Snippet: Effects of the different fruit extracts on the viability of the colon cancer cell line HCT116 and the prostate cancer cell line DU145 . DU145 (A) or HCT116 (B) were seeded in a 12-well plate and allowed to grow for 12 h. Then, 500 μg/mL of PBS-resuspended extract derived from the pulp, seeds, and skin of Meliccocus oliviformis , Nephelium lappaceum , and Manilkara zapota were added. 24h later, cell viability was assessed using the MTT assay. Data shown are the average ± SD of three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, one-way ANOVA followed by Tukey's multiple comparison test.

Article Snippet: The HCT116 (colic), and DU145 (prostatic) human cancer cell lines were purchased from ATCC (American Type Culture Collection).

Techniques: Derivative Assay, MTT Assay, Comparison

Journal: Discover Oncology

Article Title: Ferroptotic resistance involved in PTEN-loss prostate cancer progression

doi: 10.1007/s12672-025-03990-2

Figure Lengend Snippet: Erastin induced ferroptosis of prostate cancer cells. A Viability of DU145, LNCaP and PC3 cells exposed to various concentrations of Erastin for 72 h. n = 3, *** p < 0.001. B Dose effect of Erastin on cellular lipid ROS accumulation by using DCF-DA probe in DU145 cells incubated with Erastin for 24 h. n = 3, ** p < 0.01, versus to control. C Time course effect of Erastin on cellular lipid ROS accumulation by using DCF-DA probe in DU145 cells incubated with 1 µM Erastin. n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus to control. D and E Colony formation assay of DU145 exposed to Erastin, RSL3, liproxstain-1 or combo as indicated for 72 h. ** p < 0.01, *** p < 0.001versus to control; ## p < 0.01, ### p < 0.001versus to Erastin or RSL3 alone. F Visualization of DU145 cells viability under exposure to Erastin (8 µM), deferoxamine (DFO, 12.5 µM) and combo for 72 h. The magnification is 100×

Article Snippet: Prostate cancer cell lines DU145, PC3 and LNCaP were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Incubation, Control, Colony Assay

Journal: Discover Oncology

Article Title: Ferroptotic resistance involved in PTEN-loss prostate cancer progression

doi: 10.1007/s12672-025-03990-2

Figure Lengend Snippet: PTEN loss led to ferroptotic resistance in prostate cancer cells. A and B Western blot analysis of PTEN expression in DU145, PC3, LNCaP and DU145 PTEN knockdown cells. C and D Viability of DU145 PTEN deficient cells exposed to various concentrations of Erastin or RSL3 for 72 h. n = 3, ** p < 0.01*** p < 0.001. E and F The left, colony formation assay of DU145 exposed to Erastin or RSL3 in DU145 PTEN deficient cells for 72 h; the right, quantity of colony was presented as mean ± SD. ** p < 0.01

Article Snippet: Prostate cancer cell lines DU145, PC3 and LNCaP were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Western Blot, Expressing, Knockdown, Colony Assay

Journal: Discover Oncology

Article Title: Ferroptotic resistance involved in PTEN-loss prostate cancer progression

doi: 10.1007/s12672-025-03990-2

Figure Lengend Snippet: PTEN loss promoted transcription of GPX4. A and B Western blots analysis of AKT, p-AKT, NRF2 and GPX4 expression in DU145, PC3, LNCaP and DU145 PTEN deficient cells. C Cell viability was determined in DU145 with PTEN deficient cells under exposure to series concentration of Erastin combined with GDC0941 (0.25 µM) for 72 h. D and E mRNA expression of GPX4 and NRF2 was determined in DU145-PTEN-KD cells by qRT-PCR, *** p < 0.001. F Measurement of ROS level in DU145-PTEN-KD cells using DCF-DA probe. *** p < 0.001

Article Snippet: Prostate cancer cell lines DU145, PC3 and LNCaP were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Western Blot, Expressing, Concentration Assay, Quantitative RT-PCR

Journal: Discover Oncology

Article Title: Ferroptotic resistance involved in PTEN-loss prostate cancer progression

doi: 10.1007/s12672-025-03990-2

Figure Lengend Snippet: Knockdown of GPX4 restored sensitivity of cells to ferroptosis inducer. A Efficacy of shRNA targeting GPX4 was determined in PTEN deficient DU145 cells by western blot. B and C Sensitivity of DU145 cells with PTEN and GPX4 double deficient to Erastin or RSL3 was determined by MTT assay, n = 3, ** p < 0.01, *** p < 0.001 vs. control. D Efficacy of shRNA targeting GPX4 was determined in LNCaP cells by western blot. E Sensitivity of LNCaP cells with GPX4 deficient to Erastin was determined by MTT assay. n = 3, ** p < 0.01, *** p < 0.001 vs. control

Article Snippet: Prostate cancer cell lines DU145, PC3 and LNCaP were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Knockdown, shRNA, Western Blot, MTT Assay, Control